badionotus, and on other species of sea cucumbers, search on these cheated variety inside Colombia could have been limited to several education on NejlepЕЎГ datovГЎnГ lokalit pro neГєplnГЅch dvouhry variety and you may shipment [dos, 16] and you will reproductive biology [17, 18]. However, there are still many holes into the degree, instance about their reproductive habits as well as how he or she is determined by parameters such heat, salinity, water, and you will restaurants access.
The present research identifies regions of the fresh reproductive biology of holothuroids I. badionotus and you can Isostichopus sp. about Santa Marta Part making use of the gonadal index (GI) and histological examination of new gonads. This research is intended to improve the newest understanding of this new biology and you will environment off ocean cucumbers indigenous to the fresh new Colombian Caribbean Water and offer a technological reason for the development of government procedures and you can preservation of this capital.
2. Material and techniques
Specimens of Isostichopus sp. was in fact compiled out-of , while specimens regarding Isostichopus badionotus have been gathered from on Taganga Bay (11° 16′ ” N– 74° 11′ ” W) and you may Rodadero Bay (11° 12′ ” N– 74° 13′ ” W) on Caribbean region of Santa Marta, Colombia (Fig 1).
It also assesses new dictate of environment and you will chemical variables (temperatures, rain, the newest part of natural carbon blogs regarding the deposit) on the reproductive biology and you can analyzes the dwelling of its communities (volume out-of length and you may lbs, gender ratio, imply dimensions and you may lbs at first sexual readiness, fecundity)
Box into the inset A beneficial try magnified within the inset B; container in inset B is actually magnified in the primary committee. Source: Pointers base INVEMAR.
Due to the wide variety of molds and colors that i. badionotus have, this research focused on the fresh distinct organisms with prominent morphotype of the variety within the analysis area, explained from the Vergara et al. once i. badionotus morphotype II.
Month-to-month fourteen so you can 20 folks of for each and every morphotype (I. badionotus and you can Isostichopus sp.) have been compiled by snorkeling. For this specific purpose, a set of snorkelers swam an excellent transect 5 m wider because of the as much as a lot of meters long, twice. The initial journey was created bordering this new coast line and the 2nd far away of about ten meters from the coastline range. The maximum search breadth was 10 yards getting logistical and you may security causes and though it’s likely that there are people at better length and you may depth, this might not analyzed in the modern investigation. Into the October, no stuff was made because of the passage through of Hurricane Matthew, and that impacted the new Colombian Caribbean during that month. All of the water cucumbers was privately packed from inside the plastic bags having seawater and you can transferred to the Aquaculture Laboratory out of Universidad del Magdalena. This new depth from bring of every personal additionally the kind of base they occupied was filed (classified as rocky, sandy, coral, rock-red coral, rock-sand, rock-sand-coral). At the same time, facial skin sediment products (having a density maybe not higher than 10 mm) had been pulled around the individuals dictate the latest part of all-natural carbon dioxide stuff playing with a size spectrometer, Euro EA 3000 elemental analyzer (EuroVector). Salinity and you can drinking water temperatures was recorded each month, if you find yourself overall water analysis have been obtained from the new records of one’s Simon Bolivar airport meteorological route of one’s town of Santa Marta.
The folks was in fact sacrificed regarding research by the placing them from inside the seawater from the 4°C, that was in addition to meant to reach an optimum county out-of contraction you to definitely allowed to slow down the error throughout the duration measurements, due to the capacity that they need to extend otherwise deal in the usually. Then the adopting the human anatomy specifications was basically drawn: overall muscles size (TL), using a flexible 1-m tape scale in the nearest 0.5 cm (immediately following give up because of the thermal treat this new contracted looks got a c or S figure, plus the recording contoured the body, so that the overall size ‘s the figure duration); gonad frequency playing with a graduated cylinder within nearest 0.step 1 cm 3 ; muscles wall structure pounds (BW), identified as the extra weight of the individual without viscera otherwise gonad and gonad weight (GW) during the nearby 0.step one grams. Gonad index (GI) is actually determined with the algorithm, GI = (GW/ BW)* a hundred [19, 20].